The LVEM25 offers a high-contrast, high-throughput, compact imaging solution with nanometer resolutions that has all the benefits of low voltage microscopy with no limitations. The LVEM25 is a powerful 3-in-1 electron microscope with unique investigative capabilities that include transmission electron microscopy (TEM), scanning transmission electron microscopy (STEM) and even electron diffraction (ED). Its multimodal imaging capabilities combined with the ability to switch between imaging modes without moving the sample or re-alignment of the column make the LVEM25 a truly comprehensive imaging tool.
|Imaging Modes||TEM, ED, STEM|
|Nominal accelerating voltage||25 kV|
|Resolving power||1.0 nm|
|Field of view||100–0.25 µm|
|Minimum probe size||500 nm|
|Nominal accelerating voltage||15 kV|
|Resolving power||1.3 nm|
|Maximum field of view||80×80 μm|
|Nominal accelerating voltage||10 kV|
|Resolving power||1.0 nm|
|Maximum field of view||105×105 μm|
Key Technical Features
The LVEM25 microscope is quite versatile in that it can be installed wherever electron imaging is needed. It is designed to fit in where it is most needed, creating a seamless work environment.
Automatic Coarse Focus
The LVEM25’s z axis is motorized, allowing for control of sample height from the control pad and software. This additionally allows for automatic Z-height positioning and thus auto-coarse focus.
Stage Position Recording
The Piezo actuators are able to record their current position, allowing users to trace their view path as well as mark regions of interest in the software. These positons can then be recalled at a later time.
Turbomolecular Pump With Diaphragm Pump
This airlock system pump guarantees a quick and clean sample exchange. A self-venting system allows for rapid cycle times.
3 Ion Getter Pumps
These pumps ensure a non-contaminated sample space to always provide stable imaging conditions.
25kv Field Emission Gun
The Field Emission Gun (FEG) is a low voltage Schottkey type with very high brightness and spatial coherency. This allows for strong interactions between electrons and the sample. The minimum expected lifetime of the FEG is around 2,000 hours.
A high-resolution final TEM image is projected onto the YAG Screen via the electron beam. From there, light optics take over to produce an image onto the computer screen.
The fully motorized manipulator allows a sample to be easily and precisely repositioned inside the column, allowing for imaging of the entire sample.
Imaging is a breeze with the intuitive and comfortable controls that allow adjustment on illumination (brightens sample), beam correction (beam precision), magnification (up to 1.0nm resolving power), and stage movement (point of focus).
for a more detailed look of the LVEM25, click the links to the LVEM25 Technology & Design Page,
or get a detailed view with the LVEM25 Specifications Page
LVEM25 Key Applications
The LVEM25 is predominantly well suited for labs working with thin sectioned materials, owing to its ability to work with conventionally prepared thin sectioned materials.
Researchers in the field of pathology are benefited by the strikingly high contrast imagery produced. The detailed images can assist in the diagnosis of diseases like kidney disease, muscle disorders, ciliary dysfunction, neurological disorders and viral infections, to name a few, and allows for the analysis of ultrastructural pathologic changes in various tissues. To read more on how the LVEM25 is used in pathology research applications, click here.
For labs doing important research in nanoparticle characterization, the LVEM25 is an indispensable characterization tool capable of providing necessary detail on particle size and count distributions, particle shape, surface morphology as well as filament morphology, to name a few. To read more on how the LVEM25 is used in applications, click here.
In the research of polymers, the implicit low-contrast imaging due to the polymers’ molecular composition is a non-issue where the LVEM25 is concerned, due to the ultra-high contrast provided by the low energy electron source. The LVEM25 can help to greatly understand the phase behaviour and morphology of these nanostructured Polymers. To read more on how the LVEM25 is used in polymer research applications, click here.
Understanding of the organization, function and properties within cells is the reason why researchers in cell biology use electron microscopy. The LVEM25 provides higher contrast images than conventional TEM’s, whlaborich can lend a better understanding towards the location and organization of organelles, actin filaments in the cytoskeleton, and molecular complexes such as nuclear pores. To read more on how the LVEM25 is used in cell biology research applications, click here.
TEM Mode – Digital Camera
The Zyla 5.5 Scientific CMOS camera from Andor is our camera of choice for LVEM25 applications, due to its high speed, and high sensitivity imaging performance, in a light and compact TE cooled design.
|TEM IMAGE CAPTURE|
|Sensor Size||2560x2106 pixels|
|Digitization||12 bit and 16 bit|
|Pixel Size||6.5 × 6.5 μm|
|Read Noise||1.2e -|
STEM modes – Photomultiplier
With the addition of the STEM photomultiplier, the versatile LVEM25 has the added capability of scanning transmission microscopy, which can be useful in situations where samples are stained or simply too thick to act as a conventional TEM sample.
|STEM IMAGE CAPTURE|
|Monitor||(512×512, 1024×1024, 2048×2048)|
|Saving image||up to 2048×2048 pixels|
The LVEM25 is controlled through our intuitive user interface. It allows for monitoring and adjustments to all important microscope parameters. Through the software it is possible to switch seamlessly between imaging modes. The software can also serve as a virtual keyboard, replicating most of the function of the LVEM25 operating console. Image acquisition in all modes is also carried out directly within the LVEM25 software, providing the capability for live FFT, scale bar display and measurements to be made on the live image. There is also full software control over the image detectors to make adjustments to image brightness and contrast as well as a live-histogram auto-correction and bit-depth selection in the TEM mode.
For image post-processing tasks, the LVEM25 software kit includes QCapture Pro software allowing for contrast adjustments, multiple on-image measurements with export function, spatial filtering and other useful manipulations. In any case, the images are saved in non-proprietary formats, which can be imported into your favorite image analysis software, such as ImageJ.
LVEM Tours will only be available in desktop version.
The Power to Resolve
The LVEM25 is the fastest and most powerful compact transmission electron microscope. It is versatile in that it combines 2 imaging modes into one very capable machine. The practical and intuitive design along with an impressive resolving power makes the LVEM25 a pleasure to work with in any university or research facility working in nanoscale. To request a detailed, no obligations quote today, simply click the button below and we will be happy to provide one for you.Request a Quote