In cell biology, the high contrast imaging provided by a LVEM is a major advantage. Electron microscopy in general allows for the study of the activities, functions, properties and organization of cells. This provides a deep understanding of organization and function within cells.
Electron microscopy approaches complement light microscopy by providing greater detail. Electron microscopy is the only technique with sufficient resolution to localize proteins to small membrane subdomains within the cell. Researchers can better understand the location and organization of organelles, actin filaments in the cytoskeleton, and molecular complexes such as nuclear pores.
Although there are instances where staining is desirable for diagnostic purposes, the necessity to stain samples in order to generate adequate detailed contrast cannot be viewed as advantageous. The LVEM microscopes still allows for staining as an option, yet high contrast result are acquired from samples in their inherent, natural state. The LVEM25 has the added benefit of being able to work with conventionally prepared thin sectioned materials. This provides images without the side effects often encountered such as staining artifacts or the sample crashing out by chemical reaction with heavy metals.
Electron microscopy traditionally is one of the most demanding techniques to learn. However, the simplicity of the LVEM5 and LVEM25 combined with the elimination of the staining step makes it accessible to many more researchers.
The versatile LVEM5 electron microscope operates with four distinct imaging modes; TEM, SEM, STEM and ED. This provides for comprehensive imaging and finer study. The more powerful LVEM25 is built upon the same platform as the LVEM5 and offers the same benefits, together with higher beam energy.
Whether it's in the fields of pathology or other life sciences, researchers and medical professionals are assured of top notch imaging with LVEM microscopes
LVEM5 User Testimonial
The LVEM5 in cell biology can be particularly useful both in routine microscopy and in research.
In cell biology and active morphological research of cellular and subcellular structures, this instrument opens an entirely new field. The low voltage and the high contrast that it generates allow for simplified fixation protocols. Fixation with osmium tetroxide is not required anymore to generate nice contrasted images of cell components. Furthermore, due to the fact that the electrons are not highly accelerated, penetration, scattering and transmission of electrons through the section are much more efficient and generate differences in contrasts within cell structures. Hence, substructures and heterogeneities unknown up to now can be revealed within cellular compartment that were considered homogeneous.
The instrument generates images that are identical to those generated by a conventional 80KV transmission electron microscope. The LVEM5 has the advantage of avoiding additional tissue treatments such as post-sectioning staining with uranyl acetate and lead citrate. Even without counterstaining, the tissue reveals the usual cellular structures as a standard 80KV transmission microscope due to the high contrast generated by the low voltage. Compared to standard electron microscopes, the LVEM5 has the advantage of reducing the time for tissue preparation together with avoiding several treatments with heavy metals
The LVEM5 carries a great potential for advancing our comprehension of cellular structures, composition and organization.
Dr. Moïse Bendayan
Professor of Pathology & Cell Biology
Departement de Pathologie et Biologie Cellulaire
Universite de Montréal