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STEM imaging differs from TEM in that, as opposed to the entire specimen being simultaneously illuminated by the electron beam, the beam is reduced to a much smaller diameter and scans across the specimen. This technique is useful when viewing stained samples or when the samples are thicker than that required by TEM.

 

 

 

 

    

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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